Journal: Blood Advances

Article Title: IL-33, IL-25, and TSLP induce a distinct phenotypic and activation profile in human type 2 innate lymphoid cells

doi: 10.1182/bloodadvances.2016002352

Figure Lengend Snippet: Cytokines used for in vitro culture of ILC1, ILC2, and ILC3

Article Snippet: For intracellular staining experiments, cells were plated at a density of 1000 to 2000 cells per well. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Cytokine Supplier Catalog number Concentration (ng/mL) Recombinant human IL-2 R & D Systems 202-IL-050 500 Recombinant human IL-23 R & D Systems 1290-IL-010 50 Recombinant human IL-12 R & D Systems 219-IL-025 50 Recombinant human IL-33 MedImmune N/A 500 Recombinant human IL-17E Peprotech AF-200-24 500 Recombinant human IL-1 β R & D Systems 201-LB-025 500 Recombinant human IL-7 R & D Systems 207-IL-005 10 Recombinant human TSLP R & D Systems 1398-TS-010 500 Open in a separate window Cytokines used for in vitro culture of ILC1, ILC2, and ILC3 Flow cytometry Cells were centrifuged for 10 minutes at 400 g , and supernatants collected and stored at −80°C.

Techniques: In Vitro, Concentration Assay, Recombinant

Journal: PLoS ONE

Article Title: Increase of Th17 Cell Phenotype in Kidney Transplant Recipients with Chronic Allograft Dysfunction

doi: 10.1371/journal.pone.0145258

Figure Lengend Snippet: (A) PBMCs were stained with anti-CD4 PE-cy7, anti-CD25 APC, anti-IFN-γ FITC, anti-IL-17 PE, anti-IL-4 APC and anti-Foxp3 FITC. CD4+ T cells were gated for further analysis. (B) The proportion (%) of IFN-γ + /CD4 + T cells (C) IL-4 + /CD4 + T cells (D) IL-17 + /CD4 + T cells (E) CD25 + FOXP3 + /CD4 + T cells in each patient group. * P <0.05 for each comparison. LTS, long term stable; CAD, chronic allograft dysfunction; ES, early stable; ESRD, end stage renal disease; HC, healthy control.

Article Snippet: HRPTEpiCs were stimulated with various doses of recombinant human IL-17 (rhIL-17, R&D Systems, Inc. Minneapolis, MN)(10, 50, 100 ng/ml) for 72 h. Supernatants were harvested and stored at –80°C until analysis.

Techniques: Staining, Comparison

Journal: PLoS ONE

Article Title: Increase of Th17 Cell Phenotype in Kidney Transplant Recipients with Chronic Allograft Dysfunction

doi: 10.1371/journal.pone.0145258

Figure Lengend Snippet: (A) PBMCs were stained with anti-CD4 PE-cy7, anti-CD45RA–FITC, anti-CCR7 APC and anti-IL-17 PE. CD4+ T cells were gated for further analysis. (B) The proportion (%) of T naïve /CD4 + T (CD45RA + CCR7 + /CD4 + Tcells) (C) T CM /CD4 + T (CD45RA – CCR7 + /CD4 + Tcells) (D) T EM /CD4 + T (CD45RA – CCR7 – /CD4 + Tcells) (E) After surface staining with CD45 and CCR7 mAbs, analysis of IL-17 in CD4 + T cell subsets by intracellular flow cytometry was done. (F) The proportion (%) of IL-17 + /T EM. in each patient group. * P <0.05 for each comparison. LTS, long term stable; CAD, chronic allograft dysfunction; ES, early stable; ESRD, end stage renal disease; HC, healthy control.

Article Snippet: HRPTEpiCs were stimulated with various doses of recombinant human IL-17 (rhIL-17, R&D Systems, Inc. Minneapolis, MN)(10, 50, 100 ng/ml) for 72 h. Supernatants were harvested and stored at –80°C until analysis.

Techniques: Staining, Flow Cytometry, Comparison

Journal: PLoS ONE

Article Title: Increase of Th17 Cell Phenotype in Kidney Transplant Recipients with Chronic Allograft Dysfunction

doi: 10.1371/journal.pone.0145258

Figure Lengend Snippet: (A) PBMCs were stained with anti-CD4 PE-cy7, anti-CCR4 PE, anti-CCR6 APC and anti-IL-17 FITC. CD4+ T cells were gated for further analysis. (B) The proportion (%) of CCR4 + CCR6 – /CD4 + T cells (C) CCR4 – CCR6 + /CD4 + T cells (D) CCR4 + CCR6 + /CD4 + T cells in each patient group. (E) After surface staining with anti-CD4, CCR4 and CCR6 mAbs, analysis of IL-17 in CD4 + T cell subsets by intracellular flow cytometry was done. (F) The proportion (%) of IL-17 + /CCR4 + CCR6 + CD4 + T cells in each patient group. * P <0.05 for each comparison. LTS, long term stable; CAD, chronic allograft dysfunction; ES, early stable; ESRD, end stage renal disease; HC, healthy control.

Article Snippet: HRPTEpiCs were stimulated with various doses of recombinant human IL-17 (rhIL-17, R&D Systems, Inc. Minneapolis, MN)(10, 50, 100 ng/ml) for 72 h. Supernatants were harvested and stored at –80°C until analysis.

Techniques: Staining, Flow Cytometry, Comparison

Journal: PLoS ONE

Article Title: Increase of Th17 Cell Phenotype in Kidney Transplant Recipients with Chronic Allograft Dysfunction

doi: 10.1371/journal.pone.0145258

Figure Lengend Snippet: The expression of (A) IL-1beta (B) RAGE (C) HMGB1 mRNA was measured using real-time PCR. In addition, concentrations of (D) IL-17 (E) IL-33 and (F) RAGE were determined using ELISA in the serum of LTS and CAD patient group. Bars show the means. * P <0.05 vs. LTS. CAD, chronic allograft dysfunction; LTS, long term stable.

Article Snippet: HRPTEpiCs were stimulated with various doses of recombinant human IL-17 (rhIL-17, R&D Systems, Inc. Minneapolis, MN)(10, 50, 100 ng/ml) for 72 h. Supernatants were harvested and stored at –80°C until analysis.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Increase of Th17 Cell Phenotype in Kidney Transplant Recipients with Chronic Allograft Dysfunction

doi: 10.1371/journal.pone.0145258

Figure Lengend Snippet: Renal tubular epithelial cell were cultured with rhIL-17 (0, 10, 50, or 100 ng/ml) for 72 hours, and the production of (A) IL-6 and (B) IL-8 was measured using ELISA. Renal tubular epithelial cell were cultured with rhIL-17 (0, 10, 50, or 100 ng/ml) for 72 hours, and the expression of (C) ACTA-2 and (D) CTGF mRNA, relative to β-actin , was measured using real-time polymerase chain reaction. Bars show the means. *P<0.05 vs. Nil, † P<0.05 vs. IL-17 10.

Article Snippet: HRPTEpiCs were stimulated with various doses of recombinant human IL-17 (rhIL-17, R&D Systems, Inc. Minneapolis, MN)(10, 50, 100 ng/ml) for 72 h. Supernatants were harvested and stored at –80°C until analysis.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Expressing, Real-time Polymerase Chain Reaction

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: IL-17A promotes fatty acid uptake through the IL-17A/IL-17RA/p-STAT3/FABP4 axis to fuel ovarian cancer growth in an adipocyte-rich microenvironment

doi: 10.1007/s00262-019-02445-2

Figure Lengend Snippet: Relationship between IL-17A protein level and clinical outcomes in clinical ovarian cancer settings

Article Snippet: To examine the effect of recombinant human IL-17A (rhIL-17A, PeproTech Cat# 200-17) on the mRNA level of FABP4 in OvCa cells, OVCAR3 and A2780 cells were seeded in six-well plates at 5 × 10 5 cells per well, and then they were treated with rhIL-17A (0, 1, 10 or 50 ng/ml) for 24 h. Then the cells were harvested and homogenized for RNA extraction using TRIzol Reagent (TIANGEN BIOTECH Cat# DP430). cDNA was synthesized from the total RNA (2 μg) in a 20 μl reaction using a reverse transcription system (TIANGEN BIOTECH Cat# KR103-04).

Techniques:

Journal: Journal of Cellular and Molecular Medicine

Article Title: IL ‐17A promotes cell migration and invasion of glioblastoma cells via activation of PI 3K/ AKT signalling pathway

doi: 10.1111/jcmm.13938

Figure Lengend Snippet: The sequences of forward and reverse primers

Article Snippet: The human IL‐17A recombinant protein (rhIL‐17A, #8928), rabbit anti‐ZO‐1 polyclonal antibody (#8193), rabbit anti‐MMP‐9 polyclonal antibody (#15561), MMP‐2 antibody (#4022) the rabbit anti‐Slug polyclonal antibody (#9585), the rabbit anti‐AKT polyclonal antibody (#9272), the rabbit anti‐phospho‐AKT(Ser473) polyclonal antibody (#9271), the rabbit anti‐phospho‐PI3K polyclonal antibody (#4249), the rabbit anti‐phospho‐PI3K p85 (Tyr458)/p55 (Tyr199) polyclonal antibody (#4228), the anti‐rabbit IgG HRP‐linked antibody (#7074) and anti‐mouse IgG HRP‐linked antibody (#7076) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Sequencing

Journal: Journal of Cellular and Molecular Medicine

Article Title: IL ‐17A promotes cell migration and invasion of glioblastoma cells via activation of PI 3K/ AKT signalling pathway

doi: 10.1111/jcmm.13938

Figure Lengend Snippet: Correlation between IL‐17A expression and clinicopathological characteristics

Article Snippet: The human IL‐17A recombinant protein (rhIL‐17A, #8928), rabbit anti‐ZO‐1 polyclonal antibody (#8193), rabbit anti‐MMP‐9 polyclonal antibody (#15561), MMP‐2 antibody (#4022) the rabbit anti‐Slug polyclonal antibody (#9585), the rabbit anti‐AKT polyclonal antibody (#9272), the rabbit anti‐phospho‐AKT(Ser473) polyclonal antibody (#9271), the rabbit anti‐phospho‐PI3K polyclonal antibody (#4249), the rabbit anti‐phospho‐PI3K p85 (Tyr458)/p55 (Tyr199) polyclonal antibody (#4228), the anti‐rabbit IgG HRP‐linked antibody (#7074) and anti‐mouse IgG HRP‐linked antibody (#7076) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing